Oxidative Stress Associated Senescence in dermal Papilla Cells of Men with Androgenetic Alopecia.

Oxidative Stress Associated Senescence in dermal Papilla Cells of Men with Androgenetic Alopecia.

J Invest Dermatol. 2015 Feb 3.

Dermal papilla cells (DPC) taken from male androgenic alopecia (AGA) patients undergo premature senescence in vitro in association with expression of p16INK4a suggesting that DPC from balding scalp are more sensitive to environmental stress than non-balding cells. As one of the major triggers of senescence in vitro stems from the cell "culture shock" due to oxidative stress we have further investigated the effects of oxidative stress on balding and occipital scalp DPC. Patient matched DPC from balding and occipital scalp were cultured at atmospheric (21%) or physiologically normal (2%) O2. At 21% O2 DPC showed flattened morphology and a significant reduction in mobility, population doubling, increased levels of ROS and senescence associated β-Gal activity and increased expression of p16INK4a and pRB. Balding DPC secreted higher levels of the negative hair growth regulators TGF-β1 and -β2 in response to H2O2 but not cell culture associated oxidative stress. Balding DPC had higher levels of catalase and total glutathione but appear to be less able to handle oxidative stress compared to occipital DPC. These in vitro findings suggest there may be a role for oxidative stress in the pathogenesis of AGA both in relation to cell senescence and migration but also secretion of known hair follicle inhibitory factors.

Thank you CF