Search
Check Out Our Sponsors
Latest topics
Quercetin improves Intestinal Barrier Function
Page 1 of 1
Quercetin improves Intestinal Barrier Function
Posted: Sat May 02, 2009 10:19 pm Post subject: Quercetin enhances intestinal barrier function
Quercetin enhances intestinal barrier function through the assembly of zonula [corrected] occludens-2, occludin, and claudin-1 and the expression of claudin-4 in Caco-2 cells.
Suzuki T, Hara H.
Division of Applied Bioscience, Research Faculty of Agriculture, Hokkaido University Kita-9, Nishi-9, Kita-ku, Sapporo 060-8589, Japan.
Dietary flavonoids provide various beneficial effects for our health. We investigated the promotive effects of quercetin and myricetin on the intestinal barrier function in human intestinal Caco-2 cell monolayers. Transepithelial electrical resistance (TER) across the monolayers increased rapidly during incubation with quercetin, peaking at 6 h. Lucifer yellow flux, a paracellular marker, was dose-dependently lower after quercetin and myricetin treatments, although quercetin exhibited a more potent effect. Immunoblot analysis of tight junction (TJ) proteins revealed that zonula occludens (ZO)-2, occludin, and claudin-1 were distributed to the actin cytoskeleton fraction by quercetin without increasing their respective whole-cell levels and this distribution was correlated with the increases in TER. The claudin-4 level was elevated by quercetin in both the cytoskeleton fraction and whole cells after 12 h. Confocal microscopy showed the assembly of claudin-1 and -4 at the TJ by quercetin. An inhibitor of protein kinase Cdelta (PKCdelta), rottlerin, enhanced the barrier function with changes in the distribution and expression of TJ proteins in a manner very similar to that of quercetin. Phosphorylation of PKCdelta indicating the enzymatic activity in the cells was decreased by quercetin after 1 h. In the kinase assay, quercetin exhibits direct inhibition of the PKCdelta isoform. This study demonstrates that quercetin enhances the intestinal barrier function through the assembly of ZO-2, occludin, and claudin-1 by inhibiting PKCdelta and the increase in claudin-4 expression has an additional role after 12 h.
PMID: 19297429
imprisoned-radical- Posts : 493
Join date : 2011-08-10
Similar topics
» Vitamin D Preserves the Intestinal Mucosal Barrier
» Zonulin and Its Regulation of Intestinal Barrier Function: The Biological Door to Inflammation, Autoimmunity, and Cancer
» chewing gum improves digestion?
» Beneficial supplements that cross blood-brain barrier (BBB)?
» Astaxanthin Improves Cycling Times
» Zonulin and Its Regulation of Intestinal Barrier Function: The Biological Door to Inflammation, Autoimmunity, and Cancer
» chewing gum improves digestion?
» Beneficial supplements that cross blood-brain barrier (BBB)?
» Astaxanthin Improves Cycling Times
Page 1 of 1
Permissions in this forum:
You cannot reply to topics in this forum
|
|
Today at 6:36 pm by grail
» Road to recovery - my own log of everything I'm currently trying for HL
Tue Apr 30, 2024 1:55 pm by JtheDreamer
» Medical Coder During C0NV!D
Sat Apr 27, 2024 4:00 pm by CausticSymmetry
» *The first scientific evidence in 2021 that viruses do not exist*
Fri Apr 26, 2024 12:44 pm by CausticSymmetry
» Potential Natural Products Regulation of Molecular Signaling Pathway in Dermal Papilla Stem Cells
Wed Apr 17, 2024 7:44 am by CausticSymmetry
» Breast Biopsy
Sun Apr 14, 2024 2:23 am by shaftless
» Sorry if brought up before but: Best topical to help aid in breaking up fibrosis?
Sat Apr 13, 2024 2:51 am by Hoppipolla
» solar eclipse on april 8
Thu Apr 11, 2024 4:04 am by shaftless
» Role and Mechanisms of Phytochemicals in Hair Growth and Health
Wed Apr 10, 2024 4:20 am by CausticSymmetry