Heat treatment increases the incidence of alopecia areata in the C3H/HeJ mouse model

*Note: I've edited the following study because it is quite long to read. Within the link provided are images of mice who suffered alopecia areata after being subjected to heat stress.

In this study, we investigated the effects of heat treatment, a physiological stress, on AA development in C3H/HeJ mice. Whereas this strain of mice are predisposed to AA at low incidence by 18 months of age, we observed a significant increase in the incidence of hair loss in heat-treated 8-month-old C3H/HeJ mice compared with sham-treated mice. Histological analysis detected mononuclear cell infiltration in anagen hair follicles, a characteristic of AA, in heat-treated mouse skin. As expected, increased expression of induced HSPA1A/B (formerly called HSP70i) was detected in skin samples from heat-treated mice. Importantly, increased HSPA1A/B expression was also detected in skin samples from C3H/HeJ mice that developed AA spontaneously. Our results suggest that induction of HSPA1A/B may precipitate the development of AA in C3H/HeJ mice. For future studies, the C3H/HeJ mice with heat treatment may prove a useful model to investigate stress response in AA.


RESULTS
Incidence of AA in heat- and sham-treated C3H/HeJ mice

All C3H/HeJ mice had a normal appearing hair coat upon arrival to our facility. In the first experiment, we investigated whether heat treatment, as an external stressor, increased the incidence of AA. Eight-month-old mice were heated on the trunk with 48.5°C circulating water for 20 min daily for 12 consecutive days (Jimenez et al. 2008). Control mice were sham-treated with room temperature water. At the end of treatment, eight of 35 (23%) heat-treated 8-month-old mice developed AA, whereas only three of 40 (7.5%) sham-treated mice developed AA (Fig.​(Fig.1a).1a). The increase in the incidence of AA in the heat-treated group was statistically significant (p < 0.0001). We also heat- or sham-treated 18-month-old mice once a week for 6 weeks (Jimenez et al. 2008). Similar hair loss pattern was observed in heat-treated mice and mice that developed AA spontaneously

Full study: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3024057/

Additionally I'd like to emphasize that thermal stress is not the only factor in the upregulation of heat shock (stress) proteins: "The cellular stress response is characterized by an elevation of heat shock proteins (HSPs) (Park et al. 2005; Voellmy 2006). HSPs are a family of stress-responsive proteins that deal with thermal and other proteotoxic stresses including ultraviolet light, heavy metal, inflammation, and oxidative stress"

So when you guys were talking about mercury fillings adding to your hair loss issues, you were most likely correct.

Xenon wrote:Additionally I'd like to emphasize that thermal stress is not the only factor in the upregulation of heat shock (stress) proteins: "The cellular stress response is characterized by an elevation of heat shock proteins (HSPs) (Park et al. 2005; Voellmy 2006). HSPs are a family of stress-responsive proteins that deal with thermal and other proteotoxic stresses including ultraviolet light, heavy metal, inflammation, and oxidative stress"

So when you guys were talking about mercury fillings adding to your hair loss issues, you were most likely correct.

Totally agree with you about HSPs. There is another trigger however: EMF overexposure (such as from cell phones).

As you pointed out, heat shock proteins have multiple triggers, and indicate cellular stress, despite being called -heat-shock.

https://www.youtube.com/watch?v=osxIMP8k1ys
https://www.youtube.com/watch?v=qj_QgFIqAdE

I flush my face during exercises, and badly react to overheating - although can handle it severally better if i have enough electrolytes in the system and especially iodine, which in my opinion has chelation like abilities while sweating (also thermo-regulative).I am AA sufferer, and have partially reversed the condition - that means i've regrown some hair that were missing. In areata condition, can't stress enough about importance of having attention on gut triggers.

Beebrox wrote:

Xenon wrote:Additionally I'd like to emphasize that thermal stress is not the only factor in the upregulation of heat shock (stress) proteins: "The cellular stress response is characterized by an elevation of heat shock proteins (HSPs) (Park et al. 2005; Voellmy 2006). HSPs are a family of stress-responsive proteins that deal with thermal and other proteotoxic stresses including ultraviolet light, heavy metal, inflammation, and oxidative stress"

So when you guys were talking about mercury fillings adding to your hair loss issues, you were most likely correct.

Totally agree with you about HSPs. There is another trigger however: EMF overexposure (such as from cell phones).

As you pointed out, heat shock proteins have multiple triggers, and indicate cellular stress, despite being called -heat-shock.

https://www.youtube.com/watch?v=osxIMP8k1ys
https://www.youtube.com/watch?v=qj_QgFIqAdE

I flush my face during exercises, and badly react to overheating - although can handle it severally better if i have enough electrolytes in the system and especially iodine, which in my opinion has chelation like abilities while sweating (also thermo-regulative).I am AA sufferer, and have partially reversed the condition - that means i've regrown some hair that were missing. In areata condition, can't stress enough about importance of having attention on gut triggers.

Thanks, Beebrox. I'll have a look at those youtube vids in a moment. This is all starting to piece together quite well now. Mechanical force is another stress protein trigger, and would further explain why over combing my scalp (applied friction) added to my hairloss woes in the past.

These videos are very good, Beebrox, I've just watched the first one. I used to study the effects of EMF's when I was a conspiracy buff, but I never knew of their connection with stress proteins per se. I'm going to check out the second talk now.

Xenon wrote:These videos are very good, Beebrox, I've just watched the first one. I used to study the effects of EMF's when I was a conspiracy buff, but I never knew of their connection with stress proteins per se. I'm going to check out the second talk now.

There are better videos from dr. Blank than what i've posted; and there is of course a way to see the whole studies. It's interesting to me, since it's really weak signal that does the job, although that amplitude has a role also.

If interested i have quite material in .pdf form to share.

Beebrox wrote:

Xenon wrote:These videos are very good, Beebrox, I've just watched the first one. I used to study the effects of EMF's when I was a conspiracy buff, but I never knew of their connection with stress proteins per se. I'm going to check out the second talk now.

There are better videos from dr. Blank than what i've posted; and there is of course a way to see the whole studies. It's interesting to me, since it's really weak signal that does the job, although that amplitude has a role also.

If interested i have quite material in .pdf form to share.

It's interesting... a gal I used to be with, who lived about 60 miles from my hometown, would continually tell me about people in her area dying of leukemia. What also startled me, was the amount of people there who suffered personality disorders or were sexual deviants. Coincidentally she lived right by some gargantuan radio / TV transmitter (the largest in England), and I remembered wondering if the EMF's from this Mordor tower were causing these problems in so many people.

And yep, I'm interested in your pdf.

Yes, in some areas some diseases have peak and are connected directly with EMF overexposure. Leukemia, mental disorders, other cancers, biotoxin related illnesses...

There is a study that deals with how much is worth a human life. If my memory serves is ~around 30000$ in average. It's easy for establishment to ignore the pro-health concerning data and put the money in the pocket while they still can. The day will come, when using smartphone will be judged the similar way that it's eating junk, smoking or breathing traffic air, IMO.

It depends on the area, but we are most all overpowered.

J Environ Pathol Toxicol Oncol. 2014;33(4):339-47.
Hsp70 is an independent stress marker among frequent users of mobile phones.

The aim of this study was to measure the serum concentrations of heat shock protein (HSP) 70 and C-reactive protein (CRP) and the expression levels of the hsp70 gene among frequent users of mobile phones (FUMPs). We enrolled 120 employees of information technology (IT)/IT enabled service companies (FUMPs; IT professionals) and 102 infrequent users of mobile phones (IFUMPs; people from non-IT professions) as controls. The serum concentrations of HSP70 and CRP were measured by enzyme-linked immunosorbant assay and hsp70 gene expression by reverse transcription polymerase chain reaction. Significantly higher concentrations of serum HSP70 (P < 0.00012) and CRP (P < 0.04) were observed among FUMPs than IFUMPs. A higher level of hsp70 gene expres​sion(fold induction) was observed among FUMPs than IFUMPs (P < 7.06 × 10-13). In contrast to the duration of exposure-dependent increase of serum concentration of CRP, the serum HSP70 concentration was found to be independent of the duration of exposure to mobile phones. Thus, the study convincingly demonstrated the role of serum HSP and CRP as systemic inflammatory biomarkers for mobile phone-induced radiation.

Xenon wrote:

Beebrox wrote:

Xenon wrote:Additionally I'd like to emphasize that thermal stress is not the only factor in the upregulation of heat shock (stress) proteins: "The cellular stress response is characterized by an elevation of heat shock proteins (HSPs) (Park et al. 2005; Voellmy 2006). HSPs are a family of stress-responsive proteins that deal with thermal and other proteotoxic stresses including ultraviolet light, heavy metal, inflammation, and oxidative stress"

So when you guys were talking about mercury fillings adding to your hair loss issues, you were most likely correct.

Totally agree with you about HSPs. There is another trigger however: EMF overexposure (such as from cell phones).

As you pointed out, heat shock proteins have multiple triggers, and indicate cellular stress, despite being called -heat-shock.

https://www.youtube.com/watch?v=osxIMP8k1ys
https://www.youtube.com/watch?v=qj_QgFIqAdE

I flush my face during exercises, and badly react to overheating - although can handle it severally better if i have enough electrolytes in the system and especially iodine, which in my opinion has chelation like abilities while sweating (also thermo-regulative).I am AA sufferer, and have partially reversed the condition - that means i've regrown some hair that were missing. In areata condition, can't stress enough about importance of having attention on gut triggers.

Thanks, Beebrox. I'll have a look at those youtube vids in a moment. This is all starting to piece together quite well now. Mechanical force is another stress protein trigger, and would further explain why over combing my scalp (applied friction)  added to my hairloss woes in the past.

i bet there's a hormetic sweet spot with shock proteins. too little down regulates critical functions, too much and stuff goes haywire. (one reason why I backed off saunas, even though i love them and their detox effects)