Analysis of the microRNA expression profile of normal human dermal papilla cells treated with 5α-dihydrotestosterone.

Mol Med Rep. 2015 Jul;12(1):1205-12. doi: 10.3892/mmr.2015.3478. Epub 2015 Mar 12.
Analysis of the microRNA expression profile of normal human dermal papilla cells treated with 5α-dihydrotestosterone.
Lee MJ1, Cha HJ1, Lim KM1, Lee OK1, Bae S1, Kim CH2, Lee KH2, Lee YN3, Ahn KJ3, An S1.
nce has demonstrated that the accumulation of 5α‑dihydrotestosterone (DHT) in dermal papilla cells (DPCs) is implicated in androgenetic alopecia. Whether this accumulation in DHT may have direct cellular effects leading to androgenetic alopecia remains to be elucidated. The present study aimed to determine whether DHT affects cell growth, cell cycle arrest, cell death, senescence and the induction of reactive oxygen species (ROS), and whether these effects are mediated by microRNA (miRNA)‑dependent mechanisms. The cell viability and cell cycle were determined, levels of ROS were examined and senescence‑associated β‑galactosidase assays were performed in normal human DPCs (nHDPCs). Furthermore, miRNA expression profiling was performed using an miRNA microarray to determine whether changes in the expression levels of miRNA were associated with the cellular effects of DHT. The results revealed that DHT decreased cell growth by inducing cell death and G2 cell cycle arrest, and by increasing the production of ROS and senescence in the nHDPCs. In addition, 55 miRNAs were upregulated and 6 miRNAs were downregulated inthe DHT‑treated nHDPCs. Bioinformatic analysis demonstrated that the putative target genes of these upregulated and downregulated miRNAs were involved in cell growth, cell cycle arrest, cell death, senescence and the production of ROS. Specifically, the target genes of five highly upregulated and downregulated miRNAs were identified and were associated with the aforementioned effects of DHT. These results demonstrated that the expression of miRNA was altered in the DHT‑treated nHDPCs and suggest the potential mechanisms of DHT‑induced cell growth repression, cell cycle arrest, cell death, senescence and induction of ROS.