Search
Check Out Our Sponsors
Latest topics
A novel compound from celery seed with a bactericidal effect against Helicobacter pylori.
Page 1 of 1
A novel compound from celery seed with a bactericidal effect against Helicobacter pylori.
J Pharm Pharmacol. 2009 Aug;61(:1067-77.
A novel compound from celery seed with a bactericidal effect against Helicobacter pylori.Zhou Y, Taylor B, Smith TJ, Liu ZP, Clench M, Davies NW, Rainsford KD.
Biomedical Research Centre, Sheffield Hallam University, Sheffield, UK.
OBJECTIVES: The aim was to purify and characterise an antimicrobial component from celery (Apium graveolens) seeds, which have been used for centuries as a herbal medicine with reported antibacterial effects. METHODS: A crude alcoholic extract of celery seeds was fractionated by organic solvent extractions, column chromatography and HPLC. Fractions were assayed for antimicrobial activity against the gastric pathogen Helicobacter pylori and other bacteria. The purified antibacterial component was characterised via MS and NMR. Preliminary investigation of its mechanism of action included morphological studies, incorporation of macromolecular precursors, membrane integrity and two-dimensional protein electrophoresis. KEY FINDINGS: The purified component, termed 'compound with anti-Helicobacter activity' (CAH), had potent bactericidal effects against H. pylori; the minimum inhibitory concentration and minimum bactericidal concentration were 3.15 microg/ml and 6.25-12.5 microg/ml, respectively. CAH (M(r) = 384.23; empirical formula C(24)H(32)O(4)) had specific inhibitory effects on H. pylori and was not active against Campylobacter jejuni or Escherichia coli. MS and NMR data were consistent with a dimeric phthalide structure. The results appeared to rule out mechanisms that operated solely by loss of membrane integrity or inhibition of protein or nucleic acid synthesis. CONCLUSIONS: CAH may be suitable for further investigation as a potent agent for treating H. pylori infections.
A novel compound from celery seed with a bactericidal effect against Helicobacter pylori.Zhou Y, Taylor B, Smith TJ, Liu ZP, Clench M, Davies NW, Rainsford KD.
Biomedical Research Centre, Sheffield Hallam University, Sheffield, UK.
OBJECTIVES: The aim was to purify and characterise an antimicrobial component from celery (Apium graveolens) seeds, which have been used for centuries as a herbal medicine with reported antibacterial effects. METHODS: A crude alcoholic extract of celery seeds was fractionated by organic solvent extractions, column chromatography and HPLC. Fractions were assayed for antimicrobial activity against the gastric pathogen Helicobacter pylori and other bacteria. The purified antibacterial component was characterised via MS and NMR. Preliminary investigation of its mechanism of action included morphological studies, incorporation of macromolecular precursors, membrane integrity and two-dimensional protein electrophoresis. KEY FINDINGS: The purified component, termed 'compound with anti-Helicobacter activity' (CAH), had potent bactericidal effects against H. pylori; the minimum inhibitory concentration and minimum bactericidal concentration were 3.15 microg/ml and 6.25-12.5 microg/ml, respectively. CAH (M(r) = 384.23; empirical formula C(24)H(32)O(4)) had specific inhibitory effects on H. pylori and was not active against Campylobacter jejuni or Escherichia coli. MS and NMR data were consistent with a dimeric phthalide structure. The results appeared to rule out mechanisms that operated solely by loss of membrane integrity or inhibition of protein or nucleic acid synthesis. CONCLUSIONS: CAH may be suitable for further investigation as a potent agent for treating H. pylori infections.
_________________
My regimen
http://www.immortalhair.org/mpb-regimen
Now available for consultation (hair and/or health)
http://www.immortalhair.org/health-consultation
Similar topics
» Helicobacter pylori infection and dermatologic diseases
» Cure of alopecia areata after eradication of Helicobacter pylori: A new association?
» CausticSymmetry about that celery seed product
» Celery Seed great for ..
» Celery Seed Extract
» Cure of alopecia areata after eradication of Helicobacter pylori: A new association?
» CausticSymmetry about that celery seed product
» Celery Seed great for ..
» Celery Seed Extract
Page 1 of 1
Permissions in this forum:
You cannot reply to topics in this forum
|
|
Fri May 17, 2024 7:01 am by Atlas
» zombie cells
Sat May 11, 2024 6:54 am by CausticSymmetry
» Sandalore - could it be a game changer?
Wed May 08, 2024 9:45 pm by MikeGore
» *The first scientific evidence in 2021 that viruses do not exist*
Tue May 07, 2024 4:18 am by CausticSymmetry
» China is at it again
Tue May 07, 2024 4:07 am by CausticSymmetry
» Ways to increase adult stem cells
Mon May 06, 2024 5:40 pm by el_llama
» pentadecanoic acid
Sun May 05, 2024 10:56 am by CausticSymmetry
» Exosome Theory and Herpes
Fri May 03, 2024 3:25 am by CausticSymmetry
» Road to recovery - my own log of everything I'm currently trying for HL
Tue Apr 30, 2024 1:55 pm by JtheDreamer