Azelaic Acid, Vitamin B6 and Zinc - low dose = 90% inhibition of 5 Alpha-Reductase

These are the most powerful inhibitors of 5-Alpha Reductase known today and not only they inhibit the Type II form of the 5-Alpha Reductase which other products like Propecia® inhibit, but also the Type I form of the enzyme which is present in high concentrations in the scalp, sebaceous glands, and the skin. Additionally GLA, ALA and Oleic acid have potent anti-inflammatory properties.

There is strong scientific evidence that Azelaic Acid and Zinc are potent inhibitors of 5-Alpha-Reductase. When Azelaic acid, Vitamin B6 and Zinc Sulfate where added together at very low concentrations, 90% inhibition of 5 Alpha-Reductase activity was obtained.

Saw Palmetto has shown to effectively block DHT and is also believed to block the androgen receptors which are found on the hair follicles.

naturally derived ingredients that have shown to stimulate hair growth similarly to Minoxidil (Rogaine®). Recent studies by Japanese scientists have shown that Procyanidin Oligomers possess growth stimulation activity toward hair epithelial cells in vitro and stimulate anagen induction in hair follicles in vivo.

By combining GLA, ALA, Linoleic Acid, Azelaic acid, Vitamin B6, Zinc Sulfate and Saw Palmetto extract, creates an extremely powerful anti-DHT formula. By decreasing the DHT levels, hair follicles can grow and thicken naturally

The effects of zinc sulphate and azelaic acid on 5a-reductase activity in human skin were studied
using an in vitro assay with i,2[^H]-testosterone as substrate. When added at concentrations of
3 or 9 mmol/lj zinc was a potent inhibitor of 5a-reductase activity. At high concentrations, zinc
could completely inhibit the enzyme activity. Azelaic acid was also a potent inhibitor of 5areductase;
inhibition was detectable at concentrations as low as 0-2 mmol/1 and was complete at
3 mmol/l. An additive effect of the two inhibitors was observed. Vitamin B6 potentiated the
inhibitory effect of zinc, but not of azelaic acid, suggesting that two different mechanisms are
involved. When the three substances were added together at very low concentrations which had
been shown to be ineffective alone, 90% inhibition of 5a-reductase activity was obtained. If this
inhibition is confirmed in vivo, zinc sulphate combined with azelaic acid could be an effective
agent in the treatment of androgen related pathology of human skin.

Effect of zinc and azelaic acid on jcc-reductase activity
The effects of increasing concentrations of zinc sulphate (1-5 to 15 mmol/1) and azelaic acid (o-i
to 3 mmol/1) on 5a-reductase activity were studied separately and the results are shown in Figure
I. In both cases, there was a dose-dependent inhibition ofthe enzyme activity; 98% inhibition
was observed with 15 mmol/1 ZnS04 and with 3 mmol/1 azelaic acid. When the two compounds
were added together to the incubation medium at concentrations expected to give 50-60%
inhibition (3 mmol/1 and 05 mmol/1, respectively), 95% inhibition was observed (data not
shown).

Effect of vitamin B6 on zinc and azelaic acid inhibition of ^(x-reductase activity
The addition of vitamin B6 (o 025%) to zinc sulphate (15 or 3 mmol/1) resulted in a two-fold
increase in the inhibition ofthe enzyme activity (Fig. 2a). In contrast, vitamin B6 had no effect
on 5a-reductase activity when added alone or together with azelaic acid (Fig. 2b). These results
suggest that zinc and azelaic acid might inhibit 5a-reductase activity through two different
mechanisms.

Effect of simultaneous addition of zinc, azelaic and vitamin B6 at low concentrations
The additive effect of the three compounds was studied in order to determine minimal
concentrations which would effectively inhibit 5a-reductase activity. From the present results,
the concentrations used (0025% vitamin B6, 05 mmol/1 ZnSO4 and 01 mmol/1 azelaic acid)
were expected to have a minimal effect, or no effect at all, on the enzyme activity. Vitamin B6
o 025% and azelaic acid at o-1 mmol/1 had no effect on the enzyme activity while ZnSO4 at 05
mmol/1 gave less than 30% inhibition (Fig. 3). In contrast, when all three compounds were
added together at these concentrations, 90% inhibition of 5a-reductase activity is observed.

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