The indirect antioxidant effects of sulforaphane

I need to get on this stuff, or at least eat more broccoli...


Powerful and prolonged protection of human retinal pigment epithelial cells, keratinocytes, and mouse leukemia cells against oxidative damage: The indirect antioxidant effects of sulforaphane

Lewis B. and Dorothy Cullman Cancer Chemoprotection Center, and Department of Pharmacology and Molecular Sciences, The Johns Hopkins University School of Medicine, Baltimore, MD 21205

Abstract

Mammalian cells are equipped with elaborate systems for protection against the toxicity of reactive oxygen and nitrogen species and electrophiles that are constant dangers to the integrity of their DNA. Phase 2 enzymes (e.g., glutathione transferases, NAD(P)H:quinone reductase) and glutathione synthesis are widely recognized as playing major protective roles against electrophilic carcinogens, but their antioxidant functions have attracted far less attention. The cytotoxicities of four oxidative stressors (menadione, tert-butyl hydroperoxide, 4-hydroxynonenal, and peroxynitrite) for human adult retinal pigment epithelial cells (ARPE-19) were quantified by measuring the concentration dependence of cell death and were expressed as the median effect dose (D m) for each oxidant. After treatment of ARPE-19 cells for 24 h with 0–5 μM concentrations of sulforaphane (the powerful Phase 2 enzyme inducer isolated from broccoli), the toxicities of the oxidants were markedly reduced as shown by 1.5- to 3-fold increases in D m values. The magnitude of protection was a function of the nature of the oxidants and the concentrations of both the oxidants and sulforaphane. Protection was prolonged and persisted for several days after removal of sulforaphane before returning to control levels. The sulforaphane-dependent increases in specific activities of cytosolic quinone reductase and the glutathione levels were highly significantly correlated with the degree of protection as measured by D m values. Antioxidant protection was also demonstrated for human HaCaT keratinocytes and L1210 murine leukemia cells. It is therefore highly likely that the multifaceted and prolonged antioxidant protection provided by sulforaphane is a general phenomenon that is mediated through induction of the Phase 2 enzyme response.

Lucky13 - If you choose to eat the broccoli instead of supplement, opt for broccoli sprouts, they are far richer a source of SF than the usual route.

Thanks CS, that's have I've heard. I am going to look for some soon and have those until my next swanson order which they have very cheap sulforaphne supps. This stuff looks very promising for hair and for health. Think I might boot tocotrienols for this stuff.

im def adding broccomax soon

Lucky13 - Honestly, would ditch Swanson's or any other brand of Sulforaphane, except for Jarrow's Broccomax.

It's the only supplement aside from a very expensive competitor not worth mentioning that has enough sulforaphane to matter. If you buy anything else, you could be throwing your money away.

CausticSymmetry what about that one from Natures Herbs? Their celery extract is pretty good ..

North-Europe - Nature's Herb does make a decent celery seed extract, although jdp710 has mentioned a few times that Natural Factors version is more potent--although it is about twice the price also. However, I suppose it depends on what effect. I pretty confident that even at a moderate dose, either brand will work for lowering uric acid levels. Natural Factors packs more of a punch in terms of generating nitric oxide though.

You are absolutely right CS, I forgot to take a look at how much sulforaphane was in those capsules..400mcg!! I think I was just astonished by how cheap the price was. I will try the broccomax or just see if I can manage to eat broccoli sprouts a few times a week.

Been buying Brocco Sprouts from the supermarket, as I thought it be good to have at least one supplement directly from the food.


Any idea how much I would need to eat to get a good dose of sulphoraphane? I know jdp710 has mentioned that he grows his own, any good guesses?

DBAL - 100 grams (3 ounces) of Broccoli sprouts will yield abotu 250 mg of Sulforaphane.

Regular Broccoli will yield 50 times less.

ok, that's quite a lot of brocco sprouts to be eating a day.

Perhaps Ill just get the supp!

DBAL - The broccomax supp only contains 30 mg of sulforaphane so to match that each day it would be only 12.5 g of sprouts

I remember reading somewhere that broccoli sprouts have something like 50-70mg of SGS per ounce, but is there a consensus on how much of that is actually converted into sulforaphane? I'm not sure I believe that every last mg of SGS is directly converted to sulforaphane, but mostly because that just seems too good to be true. In any case, I'm hoping to get some quality broccoli seeds soon, as I just bought a sprouter and have been looking to add things to my morning shake. Maybe some wheatgrass as well.

Another reason to supplement with broccomax.

http://oregonstate.edu/ua/ncs/archives/2010/apr/%E2%80%98epigenetic%E2%80%99-concepts-offer-new-approach-degenerative-disease

"The good news – for cancer and perhaps many other health problems – is that “HDAC inhibitors” can stop this degenerative process, and some of them have already been identified in common foods. Examples include sulforaphane in broccoli, indole-3-carbinol in cruciferous vegetables, and organosulfur compounds in vegetables like garlic and onions. Butyrate, a compound produced in the intestine when dietary fiber is fermented, is an HDAC inhibitor, and it provides one possible explanation for why higher intake of dietary fiber might help prevent cancer."