Phlorotannins in Ecklonia cava extract inhibit matrix metalloproteinase activity.

Abstract

Matrix metalloproteinase (MMP) inhibitors have been identified as potential therapeutic candidates for metastasis, arthritis, chronic inflammation and wrinkle formation. For the first time here we report a detailed study on the inhibitory effects of phlorotannins in brown algae, Ecklonia cava (EC) on MMP activities in cultured human cell lines. A novel gelatin digestion assay could visualize complete inhibition of bacterial collagenase-1 activity at 20 microg/ml of EC extract during preliminary screening studies. Sensitive fluorometric assay revealed that EC extract can specifically inhibit both MMP-2 and MMP-9 activities significantly (P < 0.001) at 10 microg/ml. In addition, artificially induced activities of MMP-2 and MMP-9 in human dermal fibroblasts and HT1080 cells were inhibited by EC extract in a more or less similar manner to the positive control doxycycline. Even though the expression levels of MMPs differ from one cell type to the other, gelatin zymography clearly revealed that both MMP expression and activity in cells can be inhibited by EC extract. More interestingly, EC extract did not exert any cytotoxic effect even at 100 microg/ml anticipating its potential use as a safe MMP inhibitor.


http://www.ncbi.nlm.nih.gov/pubmed/16737716

isnt doxycycline supposed to grow hair/stop hair loss?
http://www.hairloss-research.org/antibiotics.html

gutted - It can, but it comes with large risks, especially long term. There isn't any reason to use the drug when there is Ecklonia Cava.

In addition, artificially induced activities of MMP-2 and MMP-9 in human dermal fibroblasts and HT1080 cells were inhibited by EC extract in a more or less similar manner to the positive control doxycycline.

is this article not saying EC is more or less similar to doxycycline?

gutted - Inhibition of MMP's is where the similarity ends.

I wrote this on my page:

The value of Ecklonia Cava (Seanol).

The most powerful inhibitor of Matrix Metalloproteinase-9 (MMP-9) is a brown algae-based polyphenol, called Ecklonia Cava. It is a brown algae which grows at a depth of about 100 feet. Seanol is effectively extracted from Ecklonia Cava and has an interconnected phenolic ring structure capable of trapping ten to 100 times the free radicals of either Green tea and Resveratrol extracts.

Life Sci. 2006 Sep 5;79(15):1436-43.

Ecklonia Cava is also known as a phlorotannin and is 40% fat soluble, this means it acts as both a water and fat soluble anti-oxidant. Ecklonia cava is by far the most versatile supplement I've ever discovered to treat hair loss. Besides it potent inhibition of MMP-9, it effects a whole array of systems.
The polyphenols of Ecklonia cava can last up to 12 hours in human metabolism, while compared to most plant-based tannins have a half-life of just 30 minutes. Ecklonia cava's oxygen radical absorbance capacity (ORAC) value, the score for anti-oxidation potential is 8,300, is much higher than most land-based polyphenols.

Ecklonia Cava is one of the most powerful and versatile plant tannins in existence. It's been found to regulate cortisol, lower blood pressure, increase growth hormone levels, lower triglycerides, provides anti-inflammatory effects by a variety of mechanisms, such as inhibition of the NF-kB inflammatory pathway which also serves to normalize blood glucose levels.

The benefits do not end there, besides its ability to penetrate the blood-brain-barrier, offering protection against oxidative stress, it also offers a significant increase in trans-cranial blood flow giving powerful release in alpha brain waves and parasympathetic nerve response. This creates a heightened sense of relaxation and mental alertness. Animal research shows positive increases in gamma-aminobutyric acid (GABA), noripenepherine and serotonin levels. A large number of human users report improved sleep. Personally, I find the beneficial effects to my hair to be the most rewarding.

You might think the benefits end there, not so fast. In addition, this phlorotannin posesses a potent anti-plasmin inhibition activity, which results in thinner blood viscosity. Generally, the thinner our blood, the longer we live. Ecklonia cava helps maintain normal blood pressure and has been comparable to the ACE inhibitor drug, enalapril.

Recently, it was found that high blood pressure is strongly associated with androgenetic hair loss.

Eur J Dermatol. 2007 May-Jun;17(3):220-2.

Ecklonia Cava has potent anti-DGAT activity. Diacylglycerol acyltransferase or DGAT is an enzyme that catalyzes triacylgycerol in adipocytes, hence the inhibition of this is currently being explored as drug targets for help with obesity. Unlike Ecklonia cava, drugs with this ability have serious side effects. Last but, certainly not least, Ecklonia Cava helps prevent premature wrinkling by increasing the enzyme activity of elastase.

Biol Pharm Bull. 2006 Aug;29(:1735-9.

So can this

"Our study indicates that the nutrient combination containing
vitamin C, L-lysine, L-proline, and EGCG inhibited MMP-2
and MMP-9, two of the digestive enzymes that cancer cells
use to invade tissues in the body. In fact, the more effective
the nutrient inhibition of cell movement was, the lower the
enzymatic activity of cancer cells"

Consider adding silica too, to amplify the vitamin c.

Although ecklonia cava lats for 12 hours, which would be superior to the above

http://www4.dr-rath-foundation.org/pdf-files/cancerresearch.pdf

Some people say this is wrong,
but consdering mmp causes hair loss,
lysine/proline are known to grow hair,

then they may also block mmp.

It's a worthy connection to make. lysine is excellent for hair, but was never connected to MMP 9 before.
it can also inhibit 5ar and converts to carnitine. it's a hair loss essential

i Will also add this

polyphenols are known to increase hair growth

http://www4.dr-rath-foundation.org/pdf-files/cancerresearch.pdf

this document would suggest, only in combo with lysine/proline vitamin c

and they help hair, by inhibiting mmp9

I have taken polyphenols, and apple polyphenols with no success. perhaps that is why - they don't work by themselves but in synergy